Journal: Heliyon

Article Title: Acyl-CoA synthetase 4 modulates mitochondrial function in breast cancer cells

doi: 10.1016/j.heliyon.2024.e30639

Figure Lengend Snippet: ACSL4 overexpression stimulates UCP2 and ANT1 mRNA levels in MCF-7 breast cancer cells. UCP2 and ANT1 mRNA expression was evaluated in MCF-7 tet-off/ACSL4 and MCF-7 tet-off control cells. (A) UCP2 and (B) ANT1 relative mRNA expression measured by real-time qPCR after RNA extraction and calculated as the 2 −ΔΔCt value (comparative Ct method) for each experimental sample (relative gene expression). Results are expressed as the mean arbitrary units ± SEM of at least three independent experiments (*p < 0.05, ***p < 0.001).

Article Snippet: The expression of NRF-1 (forward primer: 5′-GTACAAGAGCATGATCCTGGA-3′, reverse primer: 5′-GCTCTTCTGTGCGGACATC-3′), NRF-2 (forward primer: 5′-CAGCGACGGAAAGAGTATGA-3′, reverse primer: 5′-TGGGCAACCTGGGAGTAG-3′), uncoupling protein 2 (UCP2) (forward primer: 5′-TGGTCGGAGATACCAAAGCAC-3′, reverse primer: 5′-GCTCAGCACAGTTGACAATGGC-3′) and adenine nucleotide translocase (ANT1) (forward primer: 5′-TCAACGTCTCTGTCCAAGGC-3′, reverse primer: 5′-GTCAACTGTCCCCGTGTACA-3′) were assessed by real time qPCR (Mic PCR instrument, Molecular Biosystems, San Diego, CA, USA) using the SYBR Green Master Mix reagent kit (Applied Biosystems, Carlsbad, CA, USA).

Techniques: Over Expression, Expressing, Control, RNA Extraction, Gene Expression